Journal: International journal of oncology

Article Title: NAC1, a potential stem cell pluripotency factor expression in normal endometrium, endometrial hyperplasia and endometrial carcinoma.

doi: 10.3892/ijo_00000591

Figure Lengend Snippet: Figure 1. Immunoreactivity of NAC1 in normal endometrial tissues, hyperplasia and carcinoma. Intense immunoreactivity is present in the nuclei of normal endometrial glandular cells. (A) A case with strong staining of NAC1 in the early proliferative phase (upper left). (B and C) NAC1 immunointensity is negative in both hyperplasia (upper right) and grade 1 endometrial carcinoma (lower left). (D) A case with strong staining of NAC1 in grade 3 endometrial carcinoma (lower right).

Article Snippet: Immunohistochemistry was performed on deparaffinized sections using the NAC1 antibody at a dilution of 1:100 (Novus Biologicals, Littleton, CO) and an EnVision+System peroxidase kit (Dako, Carpinteria, CA).

Techniques: Staining

Journal: International journal of oncology

Article Title: NAC1, a potential stem cell pluripotency factor expression in normal endometrium, endometrial hyperplasia and endometrial carcinoma.

doi: 10.3892/ijo_00000591

Figure Lengend Snippet: Figure 2. Distribution pattern of NAC1 expression across the menstrual cycle, hyperplasia and carcinoma.

Article Snippet: Immunohistochemistry was performed on deparaffinized sections using the NAC1 antibody at a dilution of 1:100 (Novus Biologicals, Littleton, CO) and an EnVision+System peroxidase kit (Dako, Carpinteria, CA).

Techniques: Expressing

Journal: International journal of oncology

Article Title: NAC1, a potential stem cell pluripotency factor expression in normal endometrium, endometrial hyperplasia and endometrial carcinoma.

doi: 10.3892/ijo_00000591

Figure Lengend Snippet: Figure 3. Effects of NAC1 knockdown on cell proliferation and apoptosis in endometrial cancer cell lines. (A) Western blot analysis showing a higher expression level of NAC1 protein in Ishikawa, HHUA and JHEM2 cells than in HEC1B and JHEM1 cells. (B) Western blot analysis showing a significant reduction of NAC1 protein in NAC1 siRNA-treated cells compared with control siRNA-treated cells in HHUA and JHEM2 cells. NAC1 siRNA significantly reduces the cell number in Ishikawa cells. (C) NAC1 siRNA significantly reduces cell numbers in cell lines with NAC1 overexpression including Ishikawa, HHUA and JHEM2 compared with cell lines without NAC1 overexpression.

Article Snippet: Immunohistochemistry was performed on deparaffinized sections using the NAC1 antibody at a dilution of 1:100 (Novus Biologicals, Littleton, CO) and an EnVision+System peroxidase kit (Dako, Carpinteria, CA).

Techniques: Knockdown, Western Blot, Expressing, Control, Over Expression

Journal: International journal of oncology

Article Title: NAC1, a potential stem cell pluripotency factor expression in normal endometrium, endometrial hyperplasia and endometrial carcinoma.

doi: 10.3892/ijo_00000591

Figure Lengend Snippet: Figure 4. Detection of apoptotic cells. (A) The experiment was performed 72 h after NAC1 siRNA or control siRNA treatment. Treatment with NAC1 siRNA increases apoptosis of Ishikawa, HHUA, and JHEM2 cells as measured by Annexin V staining.

Article Snippet: Immunohistochemistry was performed on deparaffinized sections using the NAC1 antibody at a dilution of 1:100 (Novus Biologicals, Littleton, CO) and an EnVision+System peroxidase kit (Dako, Carpinteria, CA).

Techniques: Control, Staining

Journal: International journal of oncology

Article Title: NAC1, a potential stem cell pluripotency factor expression in normal endometrium, endometrial hyperplasia and endometrial carcinoma.

doi: 10.3892/ijo_00000591

Figure Lengend Snippet: Figure 5. Functional analysis of NAC1 expression. (A and B) Western blot analysis shows that NAC1-transfected HEC1B and JHEM1 express NAC1 protein. Anti-V5 antibody was used to detect NAC1 protein. (C and D) The NAC1 stable transfected HEC1B and JHEM1 cells demonstrate higher proliferative activity than the vector-only controls as evidenced by a time-dependent increase in cell number in low (0.5%) serum-containing medium.

Article Snippet: Immunohistochemistry was performed on deparaffinized sections using the NAC1 antibody at a dilution of 1:100 (Novus Biologicals, Littleton, CO) and an EnVision+System peroxidase kit (Dako, Carpinteria, CA).

Techniques: Functional Assay, Expressing, Western Blot, Transfection, Activity Assay, Plasmid Preparation

Journal: Modern pathology : an official journal of the United States and Canadian Academy of Pathology, Inc

Article Title: Amplification of The ch19p13.2 NACC1 Locus in Ovarian High-grade Serous Carcinoma

doi: 10.1038/modpathol.2010.230

Figure Lengend Snippet: DNA copy number at the NACC1 locus and NAC1 immunoreactivity from representative high-grade serous carcinomas. A. Two-color fluorescence in situ hybridization in two high-grade serous carcinomas shows increased signal of the ch19p13.2 probe (red fluorescence) whereas the control probe (green fluorescence) that hybridizes to a region near the ch19p12 does not shown any gain of intensity. The case on the left panel shows discrete red probe signals whereas the case on the right shows the homogeneously staining regions. The ideogram on the left illustrates the locations which hybridize to the ch19p13.2 probe (red bar) and to the ch19p12 control probe (green bar). B: Immunohistochemistry of NAC1. High-grade ovarian serous carcinomas with high levels of ch19p13.2 amplification show intense immunostaining (3+) of NAC1 in the nuclei (top panels). High-grade ovarian serous carcinomas without detectable alteration of DNA copy number at the ch19p13.2 show variable immunostaining intensity with staining scores ranging from 0 to 2+ (bottom panel). Immunostaining scores are indicated in each photomicrograph.

Article Snippet: The NAC1 mouse monoclonal antibody used for immunohistochemistry was purchased from Novus Biologicals (Littleton, CO).

Techniques: Fluorescence, In Situ Hybridization, Control, Staining, Immunohistochemistry, Amplification, Immunostaining